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blunt end cloning vector pjet1 2  (Addgene inc)


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    Structured Review

    Addgene inc blunt end cloning vector pjet1 2
    Blunt End Cloning Vector Pjet1 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+flag/NHR1-Flag+pcDNA3%2E1+(Plasmid+%2317315)/pmc12804166-56-8-12
    Average 94 stars, based on 2 article reviews
    blunt end cloning vector pjet1 2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway
    Article Snippet: Isolated cells were stimulated with 5 μg/mL phytohemagglutinin (PHA; Sigma-Aldrich, St. Louis, MO, USA) for three days in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific), 25 mM HEPES (Quality Biological), and 5 μg/mL gentamicin (Thermo Fisher Scientific). .. The plasmids pcDNA3.1(+) Flag-His-ATM wild-type (wt), pcDNA3.1(+) Flag-His-ATM kinase-dead (kd), and hATM-S1981A (Addgene, Watertown, MA, USA) were used to overexpress either wild-type or mutant forms of ATM. .. The plasmid PEF1α-V5-His-TBK1, constructed from the empty vector pEF1α (Thermo Fisher Scientific), was used to overexpress TBK1 for co-immunoprecipitation assays.

    Transfection:

    Article Title: Manganese mediates antiviral effects by driving an ATM -TBK1 phosphorylation signaling pathway.
    Article Snippet: .. Cells were then transfected with 6 μg of the following plasmids: pcDNA3.1(+) Flag-His-ATM (wild-type) (Addgene) and/or PEF6/V5-His-TBK1 pEF6/V5-HisTBK1. .. After 48 hours, cells were treated with 100 μM MnCl2 (Sigma-Aldrich).

    Article Title: Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway
    Article Snippet: .. Cells were then transfected with 6 μg of the following plasmids: pcDNA3.1(+) Flag-His-ATM (wild-type) (Addgene) and/or PEF6/V5-His-TBK1. .. After 48 hours, cells were treated with 100 μM MnCl2 (Sigma-Aldrich).

    Article Title: Manganese mediates antiviral effects by driving an ATM -TBK1 phosphorylation signaling pathway
    Article Snippet: .. Cells were then transfected with 6 μg of the following plasmids: pcDNA3.1(+) Flag-His-ATM (wild-type) (Addgene) and/or PEF6/V5-His-TBK1 pEF6/V5-His-TBK1. .. After 48 hours, cells were treated with 100 μM MnCl 2 (Sigma-Aldrich).



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